Methods for inactivating viruses during a protein purification process
AssigneeEMD Millipore Corporation
InventorAlex Xenopoulos
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Abstract
The present application relates to novel and improved methods of achieving virus inactivation during a protein purification process.
Claims10
A method of inactivating one or more viruses in a Protein A eluate as the Protein A eluate flows from a Protein A affinity chromatography step to a flow-through purification step, the method comprising mixing the Protein A eluate with one or more virus inactivating agents using one or more in-line static mixers, wherein the flow of the Protein A eluate through the static mixer is in the laminar flow range comprising a Reynolds number below 100, wherein complete virus inactivation is achieved in less than 10 minutes or less than 5 minutes or less than 2 minutes or less than 1 minute.
A method for inactivating one or more viruses, the method comprising:
- a. subjecting a sample comprising a target protein to a Protein A affinity chromatography process, thereby to obtain an eluate;
- and
- b. continuously transferring the eluate to an in-line static mixer to mix one or more virus inactivating agents with the eluate for a duration of time which is equal to or less than 10 minutes, wherein the flow of the eluate through the static mixer is in the laminar flow range comprising a Reynolds number below 100, thereby to inactivate one or more viruses.
Dependencies are inferred from the claim text.