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US 9,809,798 B2Granted
Pericyte progenitors from peripheral blood
AssigneeNational University of Singapore
InventorsMichael Raghunath, Anna Blocki
Life of this patent
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Abstract
Thus, provided herein are pericyte progenitor cells (e.g., isolated pericyte progenitor cells), methods for generating pericyte progenitors in clinically relevant numbers for various applications applying macromolecular crowding during cell culture, and methods of using the pericyte progenitor cells.
Claims21
A method of obtaining pericyte progenitor cells comprising:
- a) culturing blood cells with a matrix to which cells can attach, and one or more carbohydrate-based hydrophilic macromolecules, each of the one or more of the macromolecules has a molecular weight from about 50 kDa to about 1000 kDa, and wherein the one or more macromolecules are present at a concentration from about 2.5 mg/ml to about 100 mg/ml, thereby producing a cell culture;
- and
- b) maintaining the cell culture under conditions in which pericyte progenitor cells are generated in the cell culture, wherein the pericyte progenitor cells express platelet-derived growth factor receptor-β (PDGFR-β) and neuron-glial antigen 2 (NG2),
- c) thereby obtaining pericyte progenitor cells.
1 more element
Dependencies are inferred from the claim text.