Targeting of podoplanin with lectin for use in the prevention and treatment of cancer
OwnerGary S. Goldberg, Yongquan Sheninventors
Life of this patent
Estimating the term…
Abstract
Lectin compositions and methods for reducing tumor cell growth and preventing or treating cancer are provided.
Claims24
A chimeric molecule comprising a lectin fused to at least one heterologous polypeptide selected from the group consisting of an epitope tag, an immunoglobulin, and a combination thereof, wherein the lectin comprises SEQ ID NO:23.
A method of targeting Pdpn expressed on a cell comprising:
- providing a cell culture comprising tumor cells and/or normal cells;
- subjecting said cells to sufficient amount of an isolated lectin consisting of the amino acid sequence of SEQ ID NO:
- 23;
- allowing sufficient amount of time for said lectin to bind cellular Pdpn thereby forming a lectin-Pdpn molecular complex comprising at least a portion of the amino acid sequence set forth in the SEQ ID NO:
- 23 and wherein formation of said complex inhibits the activity of Pdpn.
A method for inhibiting cancer cell growth comprising identifying cells overexpressed with Pdpn and contacting said cells with an effective amount of lectin that binds sialic acid consisting of an amino acid sequence as set forth in SEQ ID NO:
- 23, thereby reducing tumor cell growth.
A pharmaceutical composition comprising an isolated lectin that binds sialic acid wherein the lectin consists of the amino acid sequence as set forth in SEQ ID NO:
- 23 in a mixture with a pharmaceutically acceptable carrier comprising a flavor additive, a lubricant, a binder, a preservative, or an encapsulating material.
Dependencies are inferred from the claim text.