Hypericin albumin nanoparticle-Escherichia coli serum antibody complex and preperation method and application thereof

A hypericin albumin nanoparticle-Escherichia coli serum antibody complex is characterized by being prepared through the following steps:

• 1) mixing hypericin albumin nanoparticles and rabbit anti-Escherichia coli to obtain a mixed liquor;
• 2) adding the mixed liquor from Step 1) into ethanediol to obtain a ethanediol mixed liquor;
• 3) irradiating the ethanediol mixed liquor from Step 2) with carbon ion, placing the mixed liquor at −2° C.-5° C. for 2 hours after irradiation to obtain an irradiated ethanediol mixed liquor;
• 4) centrifuging the irradiated ethanediol mixed liquor from Step 3) at a low temperature, discarding a supernatant, and freezing and drying precipitates to obtain the hypericin albumin nanoparticle-Escherichia coli serum antibody complex,
• wherein the hypericin albumin nanoparticles and the rabbit anti-Escherichia coli are mixed according to the ratio of (800-1600) mg:(4-10) μg in Step 1);
• wherein the ratio of hypericin albumin nanoparticles:ethanediol is (800-1600) mg (12-30) g in Step 2);
• wherein the irradiation dose of carbon ion is 3-10 Gy in Step 3);
• and
• wherein the temperature of the low temperature centrifugation is −2° C.-5° C., the centrifugal rotational speed is 16500 r/min and the cryogenic temperature of precipitates is −2-0° C. in Step 4).