Peptides whose uptake in cells is controllable
AssigneeThe Regents of the University of California
InventorsRoger Tsien, Emilia Olson, Tao Jiang, Quyen Nguyen, Michael Whitney
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Abstract
Disclosed herein, in certain embodiments, is a selective transport molecule with increased in vivo circulation. In some embodiments, a selective transport molecule disclosed herein has the formula (A-X-B-C)n-M, wherein C is a cargo moiety; A is a peptide with a sequence comprising 5 to 9 consecutive acidic amino acids, wherein the amino acids are selected from: aspartates and glutatmates; B is a peptide with a sequence comprising 5 to 20 consecutive basic amino acids; X is a linker; and M is a macromolecular carrier.
Claims11
A molecule having the formula:
- (A-X-B-C)n-M wherein:
- C is a fluorescent moiety;
- A is a peptide with a sequence comprising a series of 5 glutamate residues;
- B is a peptide with a sequence comprising a series of 8 arginine residues;
- X is a cleavable linker comprising PRSFL (SEQ ID NO:
- 22);
- M is a macromolecular carrier;
- and
- n is an integer between 1 and 20;
- wherein M is bound to A or B.
A method of imaging thrombin activity in a subject, comprising imaging thrombin activity after the subject has been administered a molecule of the structure (A-X-B-C)n-M, wherein
- C is a fluorescent moiety;
- A is a peptide with a sequence comprising a series of 5 glutamate residues;
- B is a peptide with a sequence comprising a series of 8 arginine residues;
- X is a cleavable linker comprising PRSFL (SEQ ID NO:
- 22);
- M is a macromolecular carrier;
- and
- n is an integer between 1 and 20;
- wherein M is bound to A or B.
Dependencies are inferred from the claim text.