Polypeptides binding to human complement C5
AssigneeSwedish Orphan Biovitrum AB (publ)
InventorsCharlotta Berghard, Magnus Berglund, Patrik Strömberg, Malin Lindborg, Elin Gunneriusson, Joachim Feldwisch
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Abstract
The present invention relates to C5 binding polypeptides, comprising a C5 binding motif, BM, which motif consists of an amino acid sequence selected from i) EX2X3X4A X6X7EID X11LPNL X16X17X18QW X21AFIX25X26LX28D, and ii) an amino acid sequence which has at least 86% identity to the sequence defined in i), wherein the polypeptide binds to C5. The present invention moreover relates to C5 binding polypeptides for use in therapy, such as for use in treatment of a C5 related condition, and to methods of treatments.
Claims35
A C5 binding polypeptide, comprising a C5 binding motif, BM, which motif consists of an amino acid sequence selected from
- i)
- (SEQ ID NO:
- 763)
- EX2X3X4A X6X7EID X11LPNL X16X17X18QW X21AFIX25 X26LX28D,
- wherein, independently of each other,
- X2 is selected from H, Q, S, T and V;
- X3 is selected from I, L, M and V;
- X4 is selected from A, D, E, H, K, L, N, Q, R, S, T and Y;
- X6 is selected from N and W;
- X7 is selected from A, D, E, H, N, Q, R, S and T;
- X11 is selected from A, E, G, H, K, L, Q, R, S, T and Y;
- X16 is selected from N and T;
- X17 is selected from I, L and V;
- X18 is selected from A, D, E, H, K, N, Q, R, S and T;
- X21 is selected from I, L and V;
- X25 is selected from D, E, G, H, N, S and T;
- X26 is selected from K and S;
- X28 is selected from A, D, E, H, N, Q, S, T and Y;
- and
- ii) an amino acid sequence which has at least 86% identity to the sequence defined in i), wherein the polypeptide binds to C5.
A C5 binding polypeptide, which comprises the amino acid sequence:
- (SEQ ID NO:
- 764)
- K-[BM]-DPSQS XaXbLLXc EAKKL NDXdQ;
- or an amino acid sequence which has at least 79% identity to SEQ ID NO:
- 764,
- wherein
- Xa is selected from A and S;
- Xb is selected from N and E;
- Xc is selected from A, S and C;
- Xd is selected from A and S;
- and
- wherein [BM] is a C5 binding motif, which motif consists of the amino acid sequence EX2X3X4A X6X7EID X11LPNL X16X17X18QW X21AFIX25 X26LX28D (SEQ ID NO:
- 763), or an amino acid sequence which has at least 86% identity to SEQ ID NO:763,
- wherein independently of each other:
- X2 is selected from H, Q, S, T and V;
- X3 is selected from I, L, M and V;
- X4 is selected from A, D, E, H, K, L, N, Q, R, S, T and Y;
- X6 is selected from N and W;
- X7 is selected from A, D, E, H, N, Q, R, S and T;
- X11 is selected from A, E, G, H, K, L, Q, R, S, T and Y;
- X16 is selected from N and T;
- X17 is selected from I, L and V;
- X18 is selected from A, D, E, H, K, N, Q, R, S and T;
- X21 is selected from I, L and V;
- X25 is selected from D, E, G, H, N, S and T;
- X26 is selected from K and S;
- X28 is selected from A, D, E, H, N, Q, S, T and Y;
- and
- wherein the [BM] motif binds to C5.
Dependencies are inferred from the claim text.