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US 9,808,487 B2Granted
Methods for rejuvenating red blood cells
AssigneeBiomet Biologics, LLC
InventorsAlan Gray, Joel C. Higgins, Matthew D. Landrigan, Grant Cunningham
Life of this patent
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Abstract
Methods for transfusing blood to a mammalian subject. The method includes obtaining a unit of blood, rejuvenating the blood with an enhancement composition, measuring a level of 2,3-diphosphoglycerate in the blood, and delivering the blood to a subject.
Claims13
A method for treating or preventing ischemia in a subject, comprising:
- (a) obtaining a volume of blood;
- (b) performing a separation on the volume of blood, wherein a volume comprising leukocytes or plasma is separated from red blood cells;
- (c) measuring a level of a metabolic indicator in the red blood cells;
- (d) rejuvenating the red blood cells with an enhancement composition to form rejuvenated red blood cells;
- (e) measuring the level of the metabolic indicator in the rejuvenated red blood cells;
- and
- (f) delivering a volume of rejuvenated blood comprising said rejuvenated red blood cells to the subject in need thereof, wherein the quantity is based on the result of level of the metabolic indicator as measured in step (e), thereby preventing or treating ischemia in said subject.
4 more elements
A method of treating ischemia in a subject, comprising:
- (a) obtaining a volume of red blood cells;
- (b) contacting the red blood cells with an enhancement composition to form rejuvenated red blood cells;
- (c) determining a level of 2,3-diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), or both, in the rejuvenated red blood cells;
- (d) calculating a transfusion volume of rejuvenated blood comprising said rejuvenated red blood cells necessary to dose the subject to normal or higher concentration of 2,3-DPG, ATP, or both, based on the results efconcentration of 2,3-DPG, ATP, or both, as determined in step (c);
- and
- (e) administering the transfusion volume of the rejuvenated red blood cells to the subject in need thereof, thereby treating ischemia in said subject.
3 more elements
Dependencies are inferred from the claim text.