Alpha-amylase variants
AssigneeDsm IP Assets B.V.
InventorsRene Marcel De Jong, Hanna Elisabet Abbas, Evert Tjeerd Van Rij
Life of this patent
Estimating the term…
Abstract
A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue. Such a variant polypeptide may be used in the preparation of a baked product.
Claims18
A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO:
- 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids
- 16, 45, 72, 77, 94, 134, 174, 177, 178, 186, 210, 234, 283, 284, 323, 325, 652,
- said positions being defined with reference to SEQ ID NO:
- 2 wherein the alpha-amylase polypeptide variant is at least 90% identical to SEQ ID NO:
- 2, and wherein the variant has one or more altered properties as compared with a reference polypeptide having alpha-amylase activity, wherein the at least one altered property is an increased sucrose tolerance.
A method of producing an alpha-amylase polypeptide variant, which method comprises:
- a) selecting a polypeptide having alpha-amylase activity;
- b) substituting at least one amino acid residue corresponding to any of
- 16, 45, 72, 77, 94, 134, 174, 177, 178, 186, 210, 234, 283, 284, 323, 325, 652,
- said positions being defined with reference to SEQ ID NO:
- 2;
- c) optionally substituting one or more further amino acids as defined in b);
- d) preparing the variant resulting from steps a)-c);
- e) determining a property of the variant;
- and
- f) selecting a variant having an altered property in comparison a reference polypeptide having alpha-amylase activity, wherein the altered property is increased sucrose tolerance, thereby to produce an alpha-amylase polypeptide variant, wherein the alpha-amylase polypeptide variant is at least 90% identical to SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.