Arrangement for light sheet microscopy

An arrangement for light sheet microscopy comprising:

• illumination optics comprising:
• an illumination objective configured to illuminate a sample with a light sheet via an illumination beam path, when the sample is located in a medium on a sample carrier that is aligned with respect to a plane reference surface;
• wherein an optical axis of the illumination objective and the light sheet lies in a plane which forms an illumination angle not equal to zero with the normals of the plane reference surface;
• detection optics comprising:
• a detection objective in a detection beam path;
• wherein an optical axis of the detection objective forms a detection angle not equal to zero with the normals of the reference surface;
• a separating layer system comprising;
• at least one layer made of a predetermined material with a predetermined thickness which separates the medium from the illumination objective and the detection objective;
• wherein the separating layer system is configured to contact the medium by a base surface aligned parallel to the reference surface at least in a region which is accessible by the illumination objective and the detection objective for illumination and detection;
• and
• a correction lens system comprising:
• at least one correction lens configured to reduce aberrations that occur as a result of an oblique passage of illumination light, of light to be detected, or a combination thereof through interfaces of the separating layer system;
• wherein the correction lens system is arranged between the separating layer system and the illumination objective, the detection objective, or both.