US 9,804,176 B2 · Assays for vitamin D epimers

Abstract

Methods include determining an amount of an epimeric vitamin D analyte in a sample suspected of containing the epimeric vitamin D analyte. A combination is provided in an assay medium that includes the sample and a vitamin D epimer antibody that is specific for the epimeric vitamin D analyte. The assay medium is incubated under conditions for binding of the vitamin D epimer antibody to the epimeric vitamin D analyte to form an epimeric vitamin D antibody-bound complex. The amount of the epimeric vitamin D antibody-bound complex is determined and related to the amount of epimeric vitamin D analyte in the sample.

Claims12

Dependent claims

A method of determining an amount of epimeric vitamin D analyte in a sample suspected of containing the epimeric vitamin D analyte, the method comprising:

(a) providing in combination in an assay medium:
(i) the sample, and
(ii) a vitamin D epimer binding partner that is specific for the epimeric vitamin D analyte;
(b) incubating the assay medium under conditions for binding of the vitamin D epimer binding partner to the epimeric vitamin D analyte;
and
(c) determining the amount vitamin D epimer binding partner-bound complex and relating the amount of the vitamin D epimer binding partner-bound complex to the amount of the epimeric vitamin D analyte in the sample;
wherein said epimeric vitamin D analyte is 3-epi-25-hydroxyvitamin D and wherein said vitamin D epimer binding partner is an antibody that specifically binds said 3-epi-25-hydroxyvitamin D but does not bind to any detectable degree with 25-hydroxyvitamin D.

4 more elements

Dependencies are inferred from the claim text.

Assays for vitamin D epimers

Life of this patent

Assays for vitamin D epimers

Life of this patent

Abstract

Claims12