Multiplexed assay kit to evaluate the efficacy of treatment for inflammatory bowel disease

A multiplexed assay kit used to evaluate the efficacy of a treatment regimen in a patient diagnosed with inflammatory bowel disease, said kit comprising:

• (a) a multi-well assay plate comprising a plurality of wells, each well comprising at least four discrete binding domains with capture antibodies to which a plurality of biomarkers are bound, said plurality of biomarkers are selected from the group consisting of soluble Tumor Necrosis Factor receptor II (sTNFRII) and Macrophage Inflammatory Protein-1beta (MIP-1 beta), and at least two additional biomarkers selected from the group consisting of eotaxin, Soluble Interleukin-6 Receptor (sIL-6R), Monocyte Chemoattractant Protein 1 (MCP-1), Regulated on Activation, Normal T cell Expressed and Secreted protein (RANTES), wherein the multi-well assay plate is capable of communicating with a processor, the processor configured to determine a ratio of sTNFRII to MIP-1 beta in samples from said patients versus people not diagnosed with inflammatory bowel disease;
• and
• (b) in one or more vials, containers, or compartments, a set of labeled detection antibodies specific for said plurality of biomarkers.