US 9,804,171 B2 · Diagnostic method for pediatric acute-onset neuropsychiatric syndrome (PANS) and pediatric autoimmune neuropsychiatric disorder associated with streptococci infection (PANDAS)

Abstract

The present invention provides a panel of at least five clinical analyses or tests (using serum samples) to determine the risk of pediatric acute-onset neuropsychiatric syndrome (PANS) and/or pediatric autoimmune neuropsychiatric disorder associated with streptococcal infection (PANDAS) in an individual. These include enzyme linked immunosorbent assays (ELISAs) to measure antibody titers against neuronal antigens present in the brain; the neuronal antigens include lysoganglioside, tubulin, dopamine receptor D1, dopamine receptor D2, serotonin receptor 5HT2A, and serotonin receptor 5HT2C. Antibody titers against at least four of these neuronal antigens are required in the present methods; preferably antibody tiers against all of these neuronal antigens are measured. A final assay is used to quantify calcium/calmodulin-dependent protein kinase activity using a neuronal cell line. The results of these analyses or tests are then combined using an algorithm to determine whether a PANS or PANDAS diagnosis is appropriate for the individual. Depending on the diagnosis, an appropriate treatment can be determined.

Claims14

Dependent claims

A method of analyzing a sample from a patient, comprising:

a) providing a surface comprising a lysoganglioside, tubulin, dopamine receptor D1, and dopamine receptor D2 immobilized thereon;
b) contacting the surface with the sample to form an anti-lysoganglioside antibody/lysoganglioside complex, an anti-tubulin antibody/tubulin complex, an anti-dopamine receptor D1 antibody/dopamine receptor D1 complex, and an anti-dopamine receptor D2 antibody/dopamine receptor D2 complex on the surface;
c) measuring an antibody titer of each of anti-lysoganglioside antibody, anti-tubulin antibody, anti-dopamine receptor D1 antibody, and anti-dopamine receptor D2 antibody;
d) providing an assay for measuring calcium/calmodulin-dependent protein kinase II (CaM Kinase II) activity;
e) contacting the assay for measuring CaM Kinase II activity with the sample;
and
f) measuring CaM Kinase II activity.

4 more elements

Dependencies are inferred from the claim text.

Diagnostic method for pediatric acute-onset neuropsychiatric syndrome (PANS) and pediatric autoimmune neuropsychiatric disorder associated with streptococci infection (PANDAS)

Life of this patent

Diagnostic method for pediatric acute-onset neuropsychiatric syndrome (PANS) and pediatric autoimmune neuropsychiatric disorder associated with streptococci infection (PANDAS)

Life of this patent

Abstract

Claims14