Methods for single chain variable region ENOX2 antibodies for cancer detection and diagnosis
AssigneeMor-NuCo Enterprises, Inc.
InventorsD. James Morre, Brandon Eugene Hostetler, James Jinpal Kim
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Abstract
Cancers of different cellular or tissue origins express different ENOX2 cancer isoforms or combinations of isoforms and shed these proteins into the circulation. Herein are disclosed methods both for cancer detection and diagnosis of particular origin, based on the patterns and molecular weights of the isoforms which allow the identification of the cell type and or tissue of origin of the neoplasm. Relative ENOX2 amounts are proportional to tumor burden and provide a reliable measure of response to therapy and disease progression. Also provided is the amino acid sequence to which the scFv antibodies bind as the molecular basis for the specificity of the test.
Claims20
A method for detecting the presence of one or more ENOX2 transcript variants comprising:
- obtaining a sample suspected of having one or more ENOX2 transcript variants;
- electrophoretically separating the one or more ENOX2 transcript variants;
- and
- determining the presence of the one or more ENOX2 transcript variants using a single chain variable region ENOX2 antibody linked directly to alkaline phosphatase or other single step agent for detection or imaging.
An assay for detecting the presence of one or more ENOX2 transcript variant comprising:
- obtaining a sample suspected of having one or more ENOX2 transcript variants;
- electrophoretically separating the one or more ENOX2 transcript variants;
- measuring the presence of the one or more ENOX2 transcript variants in the sample using a single chain variable region ENOX2 antibody linked directly to alkaline phosphatase or other single step agent for detection or imaging;
- and
- detecting a benign to malignant transformation in the sample at least 5 years before clinical symptoms by detecting the emergence of one or more tissue-specific ENOX2 transcript variants in the sample.
A method for detecting the presence of one or more ENOX2 transcript variants to determine the cell or tissue of origin of a malignant cancer previously identified an being a cancer of unknown origin comprising:
- obtaining a sample suspected of having one or more ENOX2 transcript variants;
- electrophoretically separating the one or more ENOX2 transcript variants;
- detecting the presence of the one or more ENOX2 transcript variants using a single chain variable region ENOX2 antibody linked directly to an alkaline phosphatase or other single step agent for detection or imaging;
- and
- determining the actual origin or origins of the cancer of unknown origin, wherein the cancer is now identified as being a cancer of cervical, ovarian, prostate, breast/uterine, non-small cell lung, small cell lung, pancreatic, colon, lymphoma/leukemia or melanoma based on the molecular weight and isoelectric point of the one or more ENOX2 transcript variants, wherein the actual origin or origins of the cancer are determined at least 5 years before clinical symptoms by detecting the emergence of one or more tissue-specific ENOX2 transcript variants in the sample.
Dependencies are inferred from the claim text.