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US 9,804,152 B1Granted
Human ex vivo skin model and its use in methods of identifying modulators of skin inflammation
AssigneeThe Procter & Gamble Company
InventorsDeborah Ruth Finlay, Kevin John Mills, Charles Carson Bascom
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Abstract
Provided are methods and systems for determining functional relationships between ex vivo skin models and an inflammatory skin condition. Also provided are methods and systems for identifying modulators of inflammation of skin, as well as the use of modulators identified by such methods or systems for the preparation of cosmetic compositions, personal care products, or both.
Claims19
A method of identifying a modulator of an inflammatory response comprising:
- (a) culturing a first ex vivo human skin sample comprising an epidermal layer and a dermal layer at a temperature of about 37° C. and a humidity of about 50%, wherein the first ex vivo human skin sample is selected from the group consisting of an endogenously inflamed ex vivo human skin sample and an induced inflamed ex vivo human skin sample;
- (b) contacting the first ex vivo human skin sample with a candidate modulator;
- (c) measuring a level of a marker of inflammation in the first ex vivo human skin sample;
- (d) providing a control level for the marker of inflammation;
- and
- (e) identifying the candidate modulator as a modulator of an inflammatory response when the level of the marker of inflammation in the first ex vivo human skin sample corresponds to a change in inflammation response relative to the control level.
3 more elements
Dependencies are inferred from the claim text.