Automated method of calibrating a chromatography system and analysis of a sample

A method of calibrating a chromatography system, the method comprising:

• forming a first diluted standard solution by mixing a standard material and a diluent, the first diluted standard solution comprising:
• a first analyte having a first diluted calibrant concentration, and
• a second analyte having a second diluted calibrant concentration,
• in which the first diluted standard solution is configured to have a first predetermined calibrant ratio;
• injecting the first diluted standard solution into a chromatographic separator;
• separating the first diluted standard solution in the chromatographic separator;
• measuring, with a detector, a first peak and a second peak that have a first retention time and a second retention time, respectively;
• and
• automatically identifying
• whether the first peak corresponds to the first analyte or the second analyte, and
• whether the second peak corresponds to the first analyte or the second analyte, based on
• either an area or a height of the first peak and the second peak, and
• the first predetermined calibrant ratio;
• assigning a first analyte time interval based on one of the first retention time and second retention time that corresponds to the first analyte;
• assigning a second analyte time interval based on one of the first retention time and second retention time that corresponds to the second analyte;
• forming a second diluted standard solution by mixing the standard material and the diluent, the second diluted standard solution comprising:
• the first analyte having a third diluted calibrant concentration, and
• the second analyte having a fourth diluted calibrant concentration;
• injecting the second diluted standard solution into the chromatographic separator;
• separating the second diluted standard solution in the chromatographic separator;
• measuring, with the detector, a third peak and a fourth peak that have a third retention time and a fourth retention time, respectively;
• automatically identifying that the third peak corresponds to
• the first analyte where the third retention time falls within the first analyte time interval or
• the second analyte where the third retention time falls within the second analyte time interval;
• automatically identifying that the fourth peak corresponds to
• the first analyte where the fourth retention time falls within the first analyte time interval or
• the second analyte where the fourth retention time falls within the second analyte time interval;
• automatically calculating a first calibration equation for the first analyte based on
• an area or a height of two of the automatically identified peaks that correspond to the first analyte, the two peaks selected from the group consisting of the first peak, the second peak, the third peak, and the fourth peak, and
• the first and third diluted calibrant concentrations;
• and
• automatically calculating a second calibration equation for the second analyte based on an area or a height of two of the automatically identified peaks that correspond to the second analyte, the two peaks selected from the group consisting of the first peak, the second peak, the third peak, and the fourth peak, and
• the second and fourth diluted calibrant concentrations.