Method to enable the use of larger injection volumes and to reduce extra-column effects in chromatographic separations
OwnerMark A. Stoneinventor
Life of this patent
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Abstract
A method and apparatus for enabling larger injection volumes and for reducing extra column effects in chromatographic separations using focusing pre-columns placed upstream of the analytical, or preparative, column with applications in any chromatographic system where the requirement is that the focusing pre-column, placed upstream of the analytical column, allows larger injection volumes to be utilized and, by enabling efficient focusing of solutes onto the analytical column, results in a significant reduction of band broadening due to extra-column effects which act upstream of the analytical column.
Claims15
A column chromatography apparatus enabling larger injection volumes and minimizing extra column effects acting upstream of the column, comprising:
- an analytical column having a stationary phase with a head;
- a focusing pre-column in fluid communication with said analytical column and placed upstream of said analytical column, wherein said focusing pre-column includes a stationary phase which separates solutes from the molecules of an injection solvent and enables focusing of solutes onto said head of said analytical column;
- wherein said stationary phase of said focusing pre-column separates solutes from the molecules of injection solvent to focus the solutes onto the head of the analytical column, and wherein a portion of said stationary phase of said focusing pre-column contains sorptive materials thereby enabling said focusing pre-column to additionally function as a guard column.
A column chromatography apparatus enabling larger injection volumes and minimizing extra column effects acting upstream of a column, comprising:
- an analytical column having a stationary phase with a head;
- a focusing pre-column in fluid communication with said analytical column and placed upstream of said analytical column, wherein said focusing pre-column includes a stationary phase which separates solutes from the molecules of an injection solvent and enables focusing of solutes onto said head of said analytical column;
- wherein when used in a reversed phase mode said stationary phase of said focusing pre-column comprises particles of silica hydride to retain polar solutes in the presence of a non-polar solvent;
- and
- wherein said stationary phase of said focusing pre-column comprises silica hydride particles with a generally polar phase bonded to said silica hydride particles, thereby enabling said focusing pre-column to retain non-polar solutes.
A column chromatography apparatus enabling larger injection volumes and minimizing extra column effects acting upstream of a column, comprising:
- an analytical column having a stationary phase with a head;
- a focusing pre-column in fluid communication with said analytical column and placed upstream of said analytical column, wherein said focusing pre-column includes a stationary phase which separates solutes from the molecules of an injection solvent and enables focusing of solutes onto said head of said analytical column;
- wherein when used in a reversed phase mode said stationary phase of said focusing pre-column comprises particles of silica hydride to retain polar solutes in the presence of a non-polar solvent;
- wherein said stationary phase of said focusing pre-column comprises silica hydride particles with a generally polar phase bonded to said silica hydride particles, thereby enabling said focusing pre-column to retain non-polar solutes;
- and
- wherein said focusing pre-column has a narrower internal cross-sectional dimension than the internal cross-sectional dimension of said analytical column, such that the linear velocity on said focusing pre-column is higher than that on said analytical column, wherein the differential in linear velocity enables more efficient focusing of focuses the solutes at said head of said analytical column.
Dependencies are inferred from the claim text.