US 9,804,116 B2Granted

Method and device for detecting sample

AssigneeKabushiki Kaisha Toshiba

InventorsSadato Hongo, Hirohisa Miyamoto

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Abstract

According to one embodiment, a method for detecting a sample includes preparing a device for detecting a sample, the device including a measurement cassette, a first chamber formed by partitioning the cassette with a partition wall, a through-hole provided in the partition wall, a first electrode provided in the cassette, and a second electrode provided in the cassette, introducing a reagent and a sample containing a measuring object substance into the first chamber, introducing a conductive liquid into the second chamber, supplying current to the through-hole, allowing the measuring object substance whose surface is bound to and is covered by the tag particles via the capture substance in the first chamber to pass through the through-hole, and detecting presence of the measuring object substance.

Claims13

Dependent claims

A method for detecting a measuring object substance in a sample, the method comprising:

preparing a device for detecting a sample, the device comprising:
a measurement cassette;
a first chamber and a second chamber formed by partitioning the measurement cassette with a partition wall;
a through-hole which is provided in the partition wall and with which the first chamber and the second chamber are connected each other;
a first electrode provided in the measurement cassette located on a first chamber side, at least a part of the first electrode being located inside the first chamber;
and
a second electrode provided in the measurement cassette located on a second chamber side, at least a part of the second electrode being located inside the second chamber;
introducing a reagent and the sample into the first chamber, the sample containing the measuring object substance, and the reagent comprising a plurality of tag particles each having a dimension less than a dimension of the measuring object substance in the sample, and a capture substance which is bound to a surface of each of the tag particles and is specifically bound to a surface of the measuring object substance;
introducing a conductive liquid into the second chamber;
applying voltage between the first electrode and the second electrode;
flowing the measuring object substance from the first chamber to the second chamber through the through-hole, a surface of the measuring object substance being bound with the plurality of tag particles via the capture substance;
and
observing change in a current flowing between the first and second electrodes to detect presence of the measuring object substance in the sample.

10 more elements

A device for detecting a sample, the device comprising:

a main body;
first and second chambers which are formed by partitioning the main body with a partition wall, the first chamber having a flow channel;
a through-hole which is provided in the partition wall and with which the flow channel in the first chamber and the second chamber are connected each other;
a sample inlet which is provided in a main body portion located on an and side of the flow channel;
an outlet which is provided in a main body portion located on the other end side of the flow channel;
a first filter provided in the flow channel, wherein the first filter is located on a downstream side of flow of a sample from the sample inlet to the outlet relative to the through-hole, and retaining a reagent comprising a plurality of tag particles each having a dimension less than a dimension of the measuring object substance in the sample, and a capture substance which is bound to a surface of each of the tag particles and is specifically bound to a surface of the measuring object substance;
a first electrode provided in a main body portion located on a first chamber side, at least a part of the first electrode being located inside the first chamber;
a second electrode provided in a main body portion located on a second chamber side, at least a part of the second electrode being located inside the second chamber;
and
a desorption part which desorbs the measuring object substance from the first filter to the flow channel on an upstream side of the flow of the sample, when the measuring object substance in the sample is bound with the plurality of tag particles via the capture substance in the first filter.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application: 14/959,133Filed 4 Dec 2015
Priority: JP 2014-26389926 Dec 2014
Published: US 2016/0187335 A130 Jun 2016
Granted: 31 Oct 2017
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

G01N 27/40
Electrolytic cell components: semi-permeable membranes or partitions
G01N 15/12
By measuring electrical or magnetic effects: by observing changes in resistance or impedance across apertures when traversed by individual…
G01N 33/54346
The carrier being characterised by its particulate form: nanoparticles
G01N 33/54366
With an insoluble carrier for immobilising immunochemicals: apparatus specially adapted for solid-phase testing