Methods for degrading nucleic acid
AssigneeDNAE Group Holdings Limited
InventorSergey A. Dryga
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Abstract
Methods for degrading contaminant nucleic acid. The methods use combinations of metal ions and peroxide ions to produce a variety of oxidative species that degrade nucleic acid. Methods of the invention are useful for decontaminating laboratory equipment or solutions. After the equipment or solutions have been decontaminated, the metal ion and peroxide ion solution can be deactivated by raising the temperature to dissociate the peroxide or by binding the metal ions, e.g., with a chelating agent.
Claims26
A method for degrading nucleic acid, the method comprising:
- contacting nucleic acid with a solution comprising adding metal ions and peroxide ions to degrade the nucleic acid, such that the nucleic acid is not available for amplification;
- and
- binding the metal ions subsequent to degradation of the nucleic acid.
A method for degrading nucleic acid, such that the nucleic acid is unavailable for amplification, the method comprising:
- contacting nucleic acid with a solution comprising metal ions and peroxide ions;
- and
- heating the solution to dissociate the peroxide ions for at least about 15 minutes;
- and
- binding the metal ions subsequent to degradation of the nucleic acid.
Dependencies are inferred from the claim text.