Biomarkers for diagnosis of stroke and its causes
AssigneeThe Regents of the University of California
InventorsFrank Sharp, Boryana Stamova, Glen C. Jickling
Life of this patent
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Abstract
The present invention provides compositions and methods for the diagnosis of the occurrence and cause of stroke.
Claims8
A solid support attached to nucleic acids that hybridize to ischemia biomarkers comprising ring finger protein 141 (RNF141), C-type lectin domain family 4, member E (CLEC4E), TIMP metallopeptidase inhibitor 2 (TIMP2), putative homeodomain transcription factor 1 (PHTF1), chemokine-like factor (CKLF), Ras-related GTP binding D (RRAGD), RhoGEF and PH domain containing 4 (FGD4), cytoplasmic polyadenylation element binding protein 2 (CPEB2), similar to hCG1994130 (LOC100290882), UBX domain protein 2B (UBXN2B), ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1), bone marrow stromal cell antigen 1 (BST1), leukotriene B4 receptor (LTB4R), coagulation factor V (F5), interferon-related developmental regulator 1 (IFRD1), KIAA0319, chromatin modifying protein 1B (CHMP1B), multiple C2 domains, transmembrane 1 (MCTP1), vanin 3 (VNN3), antagonist of mitotic exit network 1 homolog (AMN1), lysosomal-associated membrane protein 2 (LAMP2), FCH domain only 2 (FCHO2), zinc finger protein 608 (ZNF608), RAS (RAD and GEM)-like GTP binding 2 (REM2), Quaking homolog, KH domain RNA binding (QKI), RNA binding motif protein 25 (RBM25), Fatty acyl CoA reductase 2 (FAR2), ST3 beta-galactoside alpha-2,3-sialyltransferase 6 (ST3GAL6), Heterogeneous nuclear ribonucleoprotein H2 (HNRNPH2), GRB2-associated binding protein 1 (GAB1), ubiquitin protein ligase E3 component n-recognin 5 (UBR5), VAMP (vesicle-associated membrane protein)-associated protein A (VAPA), phosphoglucomutase 5 (PGM5), coiled-coil domain containing 144C (CCDC144C), similar to coiled-coil domain containing 144B (LOC100134159), leukocyte cell-derived chemotaxin 2 (LECT2), short stature homeobox (SHOX), T-box 5 (TBX5), serine palmitoyltransferase, long chain base subunit 3 (SPTLC3), SNAP25-interacting protein (SNIP), RNA binding motif, single stranded interacting protein (RBMS3), prostate-specific P704P (P704P), thrombospondin, type I, domain containing 4 (THSD4), FAT tumor suppressor homolog 3 (FAT3), small nuclear ribonucleoprotein polypeptide N (SNRPN), glycine-N-acyltransferase-like 1 (GLYATL1), glutamate decarboxylase-like 1 (GADL1), coxsackie virus and adenovirus receptor (CXADR), ovo-like 2 (OVOL2), Spi-B transcription factor (Spi-1/PU.1 related) (SPIB), brix domain containing 5 (BXDC5), unc-5 homolog B (UNC5B), astrotactin 2 (ASTN2), FLJ35934, ankyrin repeat domain 28 (ANKRD28), coiled-coil domain containing 144A (CCDC144A), translocase of inner mitochondrial membrane 8 homolog A (TIMM8A), aldolase A, fructose-bisphosphate pseudogene 2 (ALDOAP2), LIM domain binding 3 (LDB3), protein tyrosine phosphatase, receptor type D (PTPRD), similar to PTPRF interacting protein binding protein 1 (LOC729222), PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), chemokine (C-C motif) receptor-like 1 (CCRL1), heterogeneous nuclear ribonucleoprotein U-like 2(HNRNPUL2), Fc receptor-like 4 (FCRL4), embryonic lethal, abnormal vision-like 2 (ELAVL2), protogenin homolog (PRTG), distal-less homeobox 6 (DLX6), forkhead box A2 (FOXA2), stearoyl-CoA desaturase 5 (SCDS), gamma-aminobutyric acid (GABA) A receptor, beta 2 (GABRB2), Glycophorin A (MNS blood group) (GYPA), LOC283027, LOC344595, Ribosomal protein L22 (RPL22), LOC100129488 and SH3-domain GRB2-like 3 (SH3GL3), wherein the solid support is attached to 100 or fewer nucleic acids.
A solid support comprising a plurality of nucleic acids that hybridize to a plurality of genes consisting of RNF141, CLEC4E, TIMP2, PHTF1, CKLF, RRAGD, CLEC4E, FGD4, CPEB2, LOC100290882, UBXN2B, ENTPD1, BST1, LTB4R, F5, IFRD1, KIAA0319, CHMP1B, MCTP1, VNN3, AMN1, LAMP2, FCHO2, ZNF608, REM2, QKI, RBM25, FAR2, ST3GAL6, HNRNPH2, GAB1, UBR5, VAPA, PGM5, CCDC144C, LOC100134159, LECT2, SHOX, TBX5, SPTLC3, SNIP, RBMS3, P704P, THSD4, FAT3, SNRPN, GLYATL1, GADL1, CXADR, OVOL2, SPIB, BXDC5, UNC5B, ASTN2, FLJ35934, ANKRD28, CCDC144A, TIMM8A, ALDOAP2, LDB3, PTPRD, LOC729222, PPFIBP1, CCRL1, HNRNPUL2, FCRL4, ELAVL2, PRTG, DLX6, FOXA2, SCD5, GABRB2, GYPA, LOC283027, LOC344595, LOC100129488, RPL22, SH3GL3, EBF1, GRM5, TSKS, ENPP2, AP3S2, LRRC37A3, C16orf68, LOC284751, IRF6, LHFP, BANK1, ARHGEF5, ZNF254, TFDP1, COL13A1, GSTK1, ADAMTSL4, P2RX5, LHFP, PIK3C2B, CHURC1, EXT2, HLA-DOA, OOEP, ZNF185, TMEM19, FCRL1, FLJ40125, ARHGEF12, CLEC18A, CD46, PTPN20A///PTPN20B, C19orf28, FLJ31945, C16orf68, SLC20A1, DOPEY2, COL13A1, LHFP, LOC284751, GRM5, LOC100144603, MTBP, SHOX2, ARHGEF5, RNF7, CLASP2, GIPC2, RANBP10, CMBL, LOC100127980, CYTH3, PROCR, LOC146880, SLC6A19, ICAM4, C12orf42, ARHGEF12, PRSS35, NT5E, LOC100271832, LHFP, NT5E, AKR1C3, CMTM1, COL13A1, SDC4, C6orf164, GPR176, BRUNOL6, SNORA68, MIF///SLC2A11, DUSP16, HIPK2, TTC7A, PPIE, GRLF1, MAP3K7IP1, LOC100129034, PER3, SMC1A, and LRRC43, USP7, MAPRE2, CSNK1G2, SAFB2, PRKAR2A, PI4KB, CRTC1, HADHA, MAP1LC3B, KATS, CDC2L1///CDC2L2, GTSE1, TCF25, CHP, LRRC40, hCG_2003956///LYPLA2///LYPLA2P1, DAXX, UBE2NL, EIF1, KCMF1, PRKRIP1, CHMP4A, TMEM184C, TINF2, PODNL1, FBXO42, LOC441258, RRP1, C10orf104, ZDHHCS, C9orf23, LRRC45, NACC1, LOC100133445///LOC115110 and PEX16.
Dependencies are inferred from the claim text.