Macromolecule delivery to nanowells
AssigneeIbis Biosciences, Inc.
InventorPhillip N. Gray
Life of this patent
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Abstract
Provided herein is technology relating to depositing and/or placing a macromolecule at a desired site for an assay and particularly, but not exclusively, to methods and systems for transporting a macromolecule such as a protein, a nucleic acid, or a protein:nucleic acid complex to an assay site, such as the bottom of a nanopore, a nanowell, or a zero mode waveguide.
Claims5
A composition for transporting a macromolecule to an assay site, wherein the composition comprises:
- a) a transport guide, wherein said transport guide is an actin filament or a microtubule with one end attached to a bottom of a zero mode waveguide well or to a nanowell;
- b) a molecular motor that binds to and moves along the transport guide wherein said molecular motor is a myosin, kinesin, or dynein;
- and
- c) a macromolecule comprising a linking domain, wherein the linking domain links the macromolecule to the molecular motor wherein said macromolecule is a DNA, a DNA polymerase or a DNA polymerase/DNA complex attached to said molecular motor wherein said molecular motor is configured to transport said DNA, said DNA polymerase or said DNA polymerase/DNA complex to the bottom of a zero mode waveguide well for single-molecule real-time DNA sequencing.
A method for delivering a macromolecule that is a DNA polymerase or DNA polymerase/DNA complex to an assay site at the bottom of a zero mode waveguide well, wherein the method comprises:
- 1) attaching an end of an actin filament or a microtubule to a bottom of a zero mode waveguide well;
- 2) providing a molecular motor that is a myosin, kinesin, or dynein for binding and traveling along the actin filament or microtubule;
- 3) linking a macromolecule that is a DNA polymerase or a DNA polymerase/DNA complex to the molecular motor;
- and
- 4) transporting the DNA polymerase or the DNA polymerase/DNA complex to the bottom of the zero mode waveguide well for single-molecule real-time DNA sequencing.
Dependencies are inferred from the claim text.