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US 9,803,226 B2Granted
Emulsion systems and emulsion-based amplification of nucleic acid
AssigneeSysmex Corporation
InventorsFrank Diehl, Philipp Angenendt
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Abstract
Methods for efficient and high throughput emulsion-based nucleic amplification are provided. In some aspects, emulsion mixtures are provided that require extremely low input energy (e.g., the energy generated by pipetting) to form emulsions that are effective for nucleic acid amplification. Efficient formulations for breaking emulsions are likewise provided.
Claims17
A method for emulsion polymerase amplification comprising:
- (a) providing a mixture comprising an aqueous phase comprising polymerase reaction mixture and an oil mixture;
- (b) emulsifying the mixture by passing the mixture through an orifice having a diameter between 2 mm and 50 μm with a velocity of 40 μl/s to 220 μl/s;
- and
- (c) subjecting the emulsion to nucleic acid amplification;
- wherein the oil mixture comprises mineral oil, diethylhexyl carbonate, polyglyceryl-4 isostearate, cetyl PEG/PPG-10/1-dimethicone, and hexyl laurate.
2 more elements
Dependencies are inferred from the claim text.