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US 9,803,221 B2Granted
Engineering microorganisms to increase ethanol production by metabolic redirection
AssigneesEnchi Corporation, Dartmouth College
InventorsYu Deng, Daniel G. Olson, Johannes Pieter van Dijken, Arthur J. Shaw, IV, Aaron Argyros, Trisha Barrett, Nicky Caiazza, Christopher D. Herring, Stephen R. Rogers, Frank Agbogbo
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Abstract
The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.
Claims22
A recombinant prokaryotic microorganism comprising:
- (a) a heterologous nucleic acid encoding a pyruvate kinase (EC 2.7.1.40);
- (b) a genetic modification that leads to the inactivation of malic enzyme (EC 1.1.1.38, EC 1.1.1.39, or EC 1.1.1.40) wherein the inactivation results from a deletion of one or more nucleic acids of an endogenous gene encoding malic enzyme or an insertion of one or more nucleic acids into an endogenous gene encoding malic enzyme;
- and
- (c) one or more genetic modifications that leads to the inactivation of one or more enzymes selected from the group consisting of:
- lactate dehydrogenase (LDH) (EC 1.1.1.27 or EC 1.1.1.28);
- phosphotransacetylase (PTA) (EC 2.3.1.8);
- and acetate kinase (ACK) (EC 2.7.2.1), wherein the inactivation results from a deletion of one or more nucleic acids of an endogenous gene encoding the one or more enzymes or an insertion of one or more nucleic acids into the endogenous gene encoding the one or more enzymes.
4 more elements
Dependencies are inferred from the claim text.