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US 9,803,219 B2Granted
Adenovirus derived helper virus for enhancing recombinant parvovirus production
AssigneeDeutsches Krebsforschungszentrum
InventorsNazim El-Andaloussi, Antonio Marchini, Jean Rommelaere, Barbara Leuchs, Max Endele
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Abstract
Described is an adenovirus derived helper virus which may comprise the adenoviral DNA sequences for E2a, S4 (orf6), the VA1 RNA gene, and the parvovirus VP capsid gene unit. Also described is a method of efficiently preparing a recombinant parvovirus (particle) which is based on the use of various adenoviral derived helper viruses/vectors.
Claims9
An isolated NB324K (NBK) cell comprising:
- (a) an adenovirus derived helper virus comprising:
- (i) adenoviral DNA sequences:
- (a) an adenoviral E2a gene;
- (b) an adenoviral E4(orf6) gene;
- and
- (c) an adenoviral VAI RNA gene;
- and
- (ii) a recombinant parvovirus VP capsid gene unit of H-1 or MVM;
- and
- (b) a recombinant parvovirus derived from H-1 or MVM that lacks the VP1 and VP2 genes,
- wherein the NBK cell produces at least two fold higher an amount of recombinant parvovirus as compared to an NBK cell which does not comprise the adenovirus derived helper virus of (a).
8 more elements
Dependencies are inferred from the claim text.