MSCA1 nucleotide sequences impacting plant male fertility and method of using same
AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsMarc Albertsen, Tim Fox, Mary Trimnell, Yongzhong Wu, Keith Lowe
Life of this patent
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Abstract
Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.
Claims36
A method of maintaining a homozygous recessive condition of a male sterile plant, the method comprising:
- (a) providing a first plant comprising homozygous recessive alleles of a Msca1 gene, wherein said plant is male sterile;
- (b) providing a second plant comprising homozygous recessive alleles of the Msca1 gene and a construct in the hemizygous condition, the construct comprising:
- (i) a first nucleotide sequence comprising the Msca1 nucleotide sequence, that when expressed in the first plant would restore male fertility;
- (ii) a second nucleotide sequence that when expressed inhibits the function or formation of viable male gametes in the second plant, such that viable male gametes are produced in the second plant containing the recessive alleles of Msca1 and that do not contain the construct;
- and
- (c) fertilizing the first plant with the male gametes of the second plant to produce progeny which maintain the homozygous recessive condition of the first plant.
A method of maintaining a homozygous recessive condition of a first plant when crossing the first plant to a second plant, the method comprising:
- (a) providing a first plant comprising homozygous recessive alleles;
- of a Msca1 gene, the expression of which results in male sterility;
- (b) providing a second plant comprising homozygous recessive alleles of the Msca1 gene and a construct in a hemizygous condition, the construct comprising:
- (i) a first nucleotide sequence comprising the Msca1 nucleotide sequence, that when expressed in the first plant would restore male fertility;
- (ii) a second nucleotide sequence selected from the group consisting of the sequence of the DAM methylase gene, Zea mays alpha amylase gene, and a cytotoxin encoding gene;
- (iii) a third nucleotide sequence operably linked to the second nucleotide sequence directing expression to plant male gametes, such that viable male gametes are produced in the second plant containing the recessive alleles, and that do not contain the construct;
- and
- (c) fertilizing the first plant with the male gametes of the second plant to produce progeny which are male sterile and maintain the homozygous recessive condition of the first plant.
A method of producing seed from a plant having female and male gametes, the method comprising:
- (a) introducing into a male sterile plant comprising homozygous recessive alleles of a Msca1 gene, a construct in the hemizygous condition comprising:
- (i) a first nucleotide sequence comprising the Msca1 nucleotide sequence;
- (ii) a second nucleotide sequence, that when expressed inhibits the function or formation of male gametes in the plant, such that the plant produces viable male gametes that do not contain the construct;
- (b) self fertilizing the plant;
- and
- (c) producing seed which contain the construct.
A method of restoring male fertility in a male sterile plant, the male sterile plant comprising homozygous recessive alleles of a Msca1 gene, the method comprising:
- introducing into said plant a construct comprising a Msca1 nucleotide sequence that is the functional complement of the homozygous recessive condition, wherein said fertility-restored plant comprises the construct in a hemizygous condition.
A method of restoring male fertility in a male sterile plant, the male sterile plant comprising homozygous recessive alleles of a Msca1 gene that impacts male fertility, the method comprising introducing into said plant a nucleotide sequence that is the functional complement of the homozygous recessive condition, wherein the nucleotide sequence is selected from the group consisting of:
- (a) SEQ ID NO:
- 1;
- (b) a nucleic acid sequence encoding the amino acid sequence comprising SEQ ID NO:
- 2;
- (c) SEQ ID NO:
- 16;
- (d) a sequence having at least 90% identity to the full length of any of the foregoing sequences wherein the sequence impacts male fertility in a plant;
- and
- (e) a sequence which hybridizes to the full length complement of any of the sequences of parts (a-c) under highly stringent conditions of a wash of 0.1 SSC, 0.1% (w/v) SDS at 65° C. wherein the sequence impacts male fertility in a plant.
Dependencies are inferred from the claim text.