Regulation of translation of heterologously expressed genes
AssigneeR1 B3 Holding B.V.
InventorsMarinus Maria Antonius Van Herpen, Jozef Maurinus Raymond Hulzink, Anton Felix Croes
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Abstract
The present invention pertains to a method of expressing a protein of interest, preferably a heterologous protein, in preferably a plant. In a preferred embodiment said plant is a doubled haploid homozygous transgenic Nicotiana tabacum plant silenced for Ntp303. Furthermore, the invention relates to said plant with or without nucleic acid constructs according to the invention. Propagation, harvest and tissue material of said transgenic Nicotiana tabacum plant is also a part of the invention.
Claims17
A nucleic acid construct comprising a first nucleotide sequence that has at least 90% nucleotide sequence identity with the nucleotide sequence of SEQ ID NO:
- 1, wherein said first nucleotide sequence comprises a nucleotide sequence that has at least 98% nucleotide sequence identity to nucleotides 104-151 of the nucleotide sequence of SEQ ID NO:
- 1 and wherein said first nucleotide sequence comprises a nucleotide sequence that comprises a repeat of at least 8 GAA units and has at least 98% nucleotide sequence identity to nucleotides 4-76 of the nucleotide sequence of SEQ ID NO:
- 1, wherein said first nucleotide sequence is operably linked to a second nucleotide sequence encoding a protein wherein said protein is heterologous and wherein said first nucleotide sequence results in an increase in translation of said second nucleotide sequence.
Dependencies are inferred from the claim text.