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US 9,803,188 B2Granted
Systems and methods for isolating nucleic acids
AssigneeIbis Biosciences, Inc.
InventorsMark W. Eshoo, Christopher Crowder
Life of this patent
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Abstract
The present disclosure relates to systems and methods for nucleic acid isolation. In particular, the present disclosure provides systems and methods for isolating nucleic acids from aqueous samples (e.g., blood or urine).
Claims14
A method of simultaneously extracting DNA and RNA from a sample, comprising:
- a) disrupting cells in said sample;
- then
- b) contacting said sample comprising said disrupted cells with a lysis solution comprising isopropanol to generate a mixture of said sample comprising said disrupted cells, said lysis solution and said isopropanol;
- then
- c) mixing said mixture;
- then
- d) incubating said mixture to generate a lysed sample;
- and then
- e) extracting nucleic acids from said lysed sample to generate extracted nucleic acids.
6 more elements
A method of simultaneously extracting DNA and RNA from a sample, comprising:
- a) bead beating said sample in the presence of a detergent and/or a proteinase to generate disrupted cells in said sample;
- then
- b) contacting said sample comprising said disrupted cells with isopropanol;
- then
- c) contacting said sample comprising said disrupted cells with said isopropanol with a lysis solution;
- then
- d) mixing said sample comprising said disrupted cells with said isopropanol with said lysis solution;
- then
- e) incubating said sample comprising said disrupted cells with said isopropanol with said lysis solution at 30° C. for one hour to generate a lysed sample;
- then
- f) extracting DNA and RNA from said lysed sample to generate extracted DNA and RNA.
8 more elements
Dependencies are inferred from the claim text.