Method for cancer targeting treatment and detection of arginine using albumin-binding arginine deiminase fusion protein

A method of treating a cancer or inhibiting arginine-dependent tumor growth in a subject comprising administering an albumin-binding arginine deiminase fusion protein to the patient weekly or biweekly to reduce the availability of circulating arginine, wherein said albumin-binding arginine deiminase fusion protein comprises a first portion comprising one or two albumin-binding domain(s) fused to a second portion comprising arginine deiminase to form the albumin-binding arginine deiminase fusion protein, and one or more linker molecules;

• the first portion being positioned far from active site of the second portion by said linker molecule such that the albumin-binding arginine deiminase fusion protein retains the activity of arginine deiminase and binds serum albumin with neither function of one portion of the fusion protein being interfered with by the other portion of the fusion protein, wherein said albumin-binding arginine deiminase fusion protein comprises a sequence selected from SEQ ID NO:
• 36, 37, 38, 39, 40 or 41, and wherein said cancer consists essentially of pancreatic cancer, leukemia, melanoma, head and neck cancer, colorectal cancer, lung cancer, breast cancer, liver cancer, nasopharyngeal cancer, esophageal cancer, prostate cancer, stomach cancer and brain cancer.