US 9,803,183 B2 · Expression vectors for an improved protein secretion

Abstract

The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a promoter sequence and a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Claims12

Dependent claims

An expression vector comprising:

a) a promoter sequence;
and
b) a nucleic acid sequence which encodes a protein comprising a signal peptide and a subtilisin,
wherein the signal peptide comprises an amino acid sequence which is at least 95% identical to the amino acid sequence specified in SEQ ID NO:
2, and
wherein the expression vector achieves improved secretion of the protein from a host cell containing the expression vector compared to secretion achieved by the expression vector having a signal peptide other than a signal peptide of b).

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/122,562Filed 25 May 2012

Priority

DE 10 2011 118 03231 May 2011

Published

US 2014/0356929 A14 Dec 2014

Granted

31 Oct 2017

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 9/50
Acting on peptide bonds: proteinases , e.g. Endopeptidases
C12N 15/625
DNA sequences coding for fusion proteins: containing a sequence coding for a signal sequence
C12N 15/70
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
C12N 15/75
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora: for Bacillus

Expression vectors for an improved protein secretion

Life of this patent

Expression vectors for an improved protein secretion

Life of this patent

Abstract

Claims12