Ionic liquid-tolerant cellulase enzymes
AssigneesThe Regents of the University of California, Sandia Corporation
InventorsJohn Gladden, Joshua Park, Steven Singer, Blake Simmons, Ken Sale
Life of this patent
Estimating the term…
Abstract
The present invention provides ionic liquid-tolerant cellulases and method of producing and using such cellulases. The cellulases of the invention are useful in saccharification reactions using ionic liquid treated biomass.
Claims15
A composition comprising:
- (i) an ionic-liquid-tolerant β-glucosidase comprising an amino acid sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO:2 and having β-glucosidase activity;
- (ii) an ionic liquid that has an imidazolium cation:
- and (iii) a lignocellulosic biomass.
A method of increasing the yield of soluble sugar from a lignocellulosic biomass, the method comprising incubating lignocellulosic biomass with a composition comprising an ionic-liquid-tolerant β-glucosidase and an ionic liquid having an imidazolium cation in an enzymatic hydrolysis reaction, wherein the ionic-liquid-tolerant β-glucosidase comprises an amino acid sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO:2 and has β-glucosidase activity.
Dependencies are inferred from the claim text.