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US 9,803,178 B2Granted
Biocatalysts for the preparation of hydroxy substituted carbamates
AssigneeCodexis, Inc.
InventorsFabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
Life of this patent
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Abstract
The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
Claims19
An engineered polynucleotide encoding a ketoreductase polypeptide capable of converting substrate compound (2), tert-butyl(1-oxo-1-phenylhex-5-yn-2-yl)carbamate, to product compound (1), tert-butyl(1-hydroxy-1-phenylhex-5-yn-2-yl)carbamate,
- wherein the ketoreductase polypeptide comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO:4 and comprises a substitution at one or more positions selected from 40, 96, 145, 195, 199, or position 206, with the proviso that when X206 is F, the sequence further comprises one or more residue differences as compared to SEQ ID NO:4 selected from:
- X7S;
- X17M;
- X17Q;
- X17R;
- X23V;
- X27L;
- X29G;
- X60I;
- X64V;
- X71P;
- X87L;
- X94A;
- X94P;
- X94S;
- X95M;
- X96L;
- X96Y;
- X105G;
- X113I;
- X122A;
- X127R;
- X131S;
- X144V;
- X145L;
- X147I;
- X147L;
- X147Q;
- X150Y;
- X152G;
- X153G;
- X157C;
- X173L;
- X195A;
- X195G;
- X196M;
- X198S;
- X199H;
- X208R;
- X216R;
- X221S;
- X243S;
- X245I;
- X249F;
- X249G;
- and X249Y.
43 more elements
Dependencies are inferred from the claim text.