Induced pluripotent stem cells with synthetic modified RNAs
AssigneeChildren's Medical Center Corporation
InventorsDerrick Rossi, Luigi Warren
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Abstract
Described herein are synthetic, modified RNAs for changing the phenotype of a cell, such as expressing a polypeptide or altering the developmental potential. Accordingly, provided herein are compositions, methods, and kits comprising synthetic, modified RNAs for changing the phenotype of a cell or cells. These methods, compositions, and kits comprising synthetic, modified RNAs can be used either to express a desired protein in a cell or tissue, or to change the differentiated phenotype of a cell to that of another, desired cell type.
Claims10
An isolated, human induced pluripotent stem (iPS) cell comprising a transfection mixture comprising synthetic modified RNAs encoding octamer-binding transcription factor 4 (Oct4), sex determining region Y-box 2 (Sox2), and Kruppel-like factor 4 (K1f4), wherein each occurrence of a cytosine is replaced with 5-methylcytosine (5mC) and each occurrence of a uracil is replaced with pseudouracil;
- wherein the iPS cell is produced by contacting an isolated human somatic fibroblast cell with the transfection mixture for a time sufficient to obtain the iPS cell.
Dependencies are inferred from the claim text.