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US 9,803,172 B2Granted
Method for inducing bacteria to enter into viable but nonculturable state
AssigneeChina Agricultural University
InventorsXiaojun Liao, Feng Zhao
Life of this patent
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Abstract
A method induces bacteria to enter into viable, but nonculturable state. The method includes subjecting a target bacterium to high pressure carbon dioxide treatment so as to make it enter into the viable, but nonculturable state, which can be demonstrated experimentally. The viable but nonculturable state can be entered quickly, such as within one hour.
Claims7
A method of inducing a target bacterium to enter into a viable but nonculturable state, comprising:
- subjecting the target bacterium to high pressure carbon dioxide treatment so as to make the target bacterium enter into the viable but nonculturable (VBNC) state, wherein conditions of the high pressure carbon dioxide treatment comprise a pressure of 5-7 MPa, a temperature of 25-37° C., and a time of 5-60 min, wherein the target bacterium is a Gram-negative bacterium.
Dependencies are inferred from the claim text.