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US 9,803,166 B2Granted
Pre-programmed non-feedback controlled continuous feeding of cell cultures
AssigneeAmgen Inc.
InventorsHenry Lin, Jeremy Bezaire
Life of this patent
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Abstract
A pre-programmed non-feedback continuous feeding method based on mass balance of the substrate in the bioreactor for use in culture growth and maintenance is provided. The disclosed method does not rely on instrument, probe or operator feedback. The method provides an efficient and effective alternative to bolus feeding.
Claims14
A method of continuously feeding a mammalian cell culture that does not employ feedback control, comprising:
- (a) providing a vessel comprising a mammalian cell culture comprising mammalian cells and media;
- (b) determining preferred values for the consumption rate (K1) of a nutrient, growth rate (K21) and growth rate (K22) of the cell culture;
- (c) providing an apparatus adapted to impart a continuous feed stream to the cell culture, wherein the apparatus comprises a controller module adapted to continuously feed the culture at a flow rate F, wherein
- F is defined as K1exp(K21t2+K22t);
- t is the duration of time from the time the feed stream is added to the bioreactor to the time when the feed stream is stopped;
- and
- K1, K21 and K22 are the values determined in (a);
- and
- (d) activating the controller module to initiate continuous feeding of the cell culture.
6 more elements
Dependencies are inferred from the claim text.