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US 9,803,164 B2Granted
Megakaryocyte and platelet production from stem cells
AssigneeNew York Blood Center, Inc.
InventorBeau W. Mitchell
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Abstract
Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.
Claims20
A platelet production device for the ex vivo production of platelets comprising:
- a fluid source, the fluid comprising a growth medium;
- a platelet production chamber in fluid communication with the fluid source, the chamber comprising a first and a second fluid flow path, the first and second fluid flow paths separated from each other within the chamber by a scaffold permeable to proplatelet processes;
- wherein the permeable scaffold allows at least a portion of the fluid in the first fluid flow path to permeate the permeable scaffold toward the second fluid flow path causing a plurality of megakaryocytes located in the first fluid path to extend their respective proplatelet processes through the permeable scaffold toward the second fluid flow path;
- wherein the platelet production device transports fluid from the fluid source into the platelet production chamber;
- and
- wherein the second fluid flow path brings the growth medium into contact with at least a portion of the permeable scaffold to remove at least some platelets from the proplatelet processes.
3 more elements
A method of growing and harvesting platelets from proplatelet processes, the method comprising:
- providing a production device for the ex vivo production of platelets, the production device comprising:
- a fluid source, the fluid comprising a growth medium;
- a platelet production chamber in fluid communication with the fluid source, the chamber comprising a first and a second fluid flow path, the first and second fluid flow paths separated from each other within the chamber by a permeable scaffold which allows at least some of the fluid in the first fluid flow path to permeate the permeable scaffold toward the second fluid flow path and is permeable to proplatelet processes, the permeable scaffold prevents a mature megakaryocyte from passing through;
- pumping the growth medium through at least one of the first and second flow paths;
- providing a plurality of megakaryocytes within the first fluid flow path so as to position the megakaryocytes against the permeable scaffold and direct their respective proplatelet processes in the direction of the second fluid flow path;
- removing the growth medium from the second flow path after it has interacted with the permeable scaffold so as to remove platelets from the proplatelet processes;
- and
- harvesting the platelets from the removed growth medium.
5 more elements
Dependencies are inferred from the claim text.