Oil globule protein and uses thereof
AssigneesBen-Gurion University of the Negev Research and Development Authority, Yeda Research and Development Co. Ltd. at the Weizmann Institute of Science
InventorsSammy Boussiba, Ehud Peled, Uri Pick, Aliza Zarka, Stefan Lou, Meira Weiss
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Abstract
An isolated novel oil globule protein encoded by a polynucleotide sequence is provided together with a composition which includes the isolated protein. A transgenic organism transformed by a polynucleotide encoding a protein which at least partially comprises the amino acid sequence of the novel oil globule protein is also provided. The invention also provides a method for producing or enhancing the production of a carotenoid such as astaxanthin, which is an oil globule constituent.
Claims11
A plant extract comprising a polynucleotide encoding the amino acid sequence set forth in SEQ ID NO:
- 1, wherein said polynucleotide is operably linked to a heterologous promoter and wherein said plant extract is obtained from a plant that does not produce astaxanthin endogenically.
An expression vector comprising a heterologous promoter operably linked to a polynucleotide encoding the amino acid sequence of SEQ ID NO:
- 1.
A transgenic plant, a transgenic seed, a transgenic heterotrophic microorganism, a transformed bacterium, or a transgenic alga comprising a polynucleotide encoding the amino acid sequence set forth in SEQ ID NO:
- 1.
A transgenic seed, produced by a transgenic plant transformed by a polynucleotide encoding the amino acid sequence set forth in SEQ ID NO:
- 1.
A method of enhancing production of oil or oil globules in a cell comprising the step of transforming said cell with a polynucleotide encoding the amino acid sequence set forth in SEQ ID NO:
- 1, thereby enhancing the production of oil or oil globules in a cell.
A method of enhancing a production of a carotenoid in a cell comprising the step of transforming said cell with a polynucleotide encoding the amino acid sequence set forth in SEQ ID NO:
- 1, thereby enhancing the production of carotenoid in a cell.
A method of enhancing production of astaxanthin in a Haematococcus pluvialis cell comprising the steps of:
- (a) transforming said cell with a composite polynucleotide comprising a polynucleotide encoding the amino acid sequence set forth in SEQ ID NO:
- 1 under the control of an inducible promoter;
- (b) growing said cell under proliferation promoting conditions;
- (c) depriving said cell of nutrients;
- and
- (d) subjecting said cell to intense light;
- thereby enhancing a production of astaxanthin in a Haematococcus pluvialis cell.
Dependencies are inferred from the claim text.