Method for stepwise separating amino acid active ingredients of Camellia nitidissima Chi

Abstract

The present invention relates to the technical field of Camellia nitidissima Chi processing and application, and provides a method for stepwise separating amino acid active ingredients of Camellia nitidissima Chi. The method comprises the following steps: taking a graphene nano material as a selective extraction, adsorption and separation carrier material; carrying out stepwise separation through stepwise controlling the pH value of Camellia nitidissima Chi concentrated solution and adjusting the isoelectric points of the amino acid active ingredients, wherein the amino acid active ingredients comprise aspartic acid, threonine, serine, glutamic acid, proline and glycine, and the pH values of the aspartic acid, the threonine, the serine, the glutamic acid, the proline and the glycine corresponding to the stepwise separated isoelectric points are less than 2.77, 5.98-6.15, 3.23-5.67, 2.78-3.21, 6.17-6.29 and 5.69-5.96. The method for stepwise separating amino acid active ingredients of Camellia nitidissima Chi has the characteristics of superior selectivity, superior separation speed, good product purity and low cost.

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