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US 9,802,822 B2Granted
Apatite pretreatment
AssigneeBio-Rad Laboratories, Inc.
InventorLarry Cummings
Life of this patent
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Abstract
Apatite pretreatment methods are provided. The method is applied to the apatite solid surface prior to first chromatographic use. In one embodiment, the method may be achieved by contacting an apatite solid surface with a phosphate buffered solution at a pH of at least about 6.5 and contacting the apatite solid surface with a solution having a hydroxide.
Claims20
A method of treating an apatite solid surface prior to first use in a chromatographic procedure, the method comprising:
- (a) providing an apatite solid surface, wherein the apatite surface has not previously been used in any chromatographic procedure and has not previously been contacted with any sample;
- (b) contacting the apatite solid surface with a phosphate buffered solution at a pH of at least about 6.5;
- and then
- (c) contacting the apatite solid surface obtained in step (b) with a solution having an alkaline metal hydroxide:
- and then
- (d) purifying protein from a sample with the apatite solid surface obtained in step (c).
3 more elements
Dependencies are inferred from the claim text.