Container for multiple particle/layer separations and particle/layer separation method using the same

A separation container, comprising:

• a separation tube receiving a sample therein;
• a first sedimentation part connected to an end portion of the tube, a particle in the sample being deposited by a centrifugal or agitating force;
• and
• a separating part provided in the tube and including at least one first and second separating layers being installed to be movable along the tube, operative to selectively open the tube in an open state and close the tube in a closed state, and movable relative to each other in the sample within the tube to adjust a spacing distance between the first and second separating layers, the first and second separating layers configured to close the tube in the closed state to form first and second boundary layers for blocking a flow of the sample therethrough respectively such that a target layer in the sample is isolated and surrounded between the first and second separating layers,
• wherein the first and second separating layers are adapted to be stationary in the open state within the tube during application of the centrifugal or agitating force, and
• wherein the first and second separating layers are operable to move toward the target layer in the open state after the centrifugation or the agitation, the first and second separating layers are operable to change from the open state into the closed state when the target layer is between the first and second separating layers, and the first and second separating layers close the tube in the closed state to block the target layer between the first and second separating layers from flowing through the first and second separating layers respectively.