Sorting chamber for microscale particles

A sorting chamber for sorting particles of a first type from particles of a second type present within a sample;

• the particles of the first type being erythrocytes and having at least one dimension smaller than the dimensions of the particles of the second type, which are lymphocytes;
• the particles of the first type and of the second type having respective major dimensions smaller than 1000 μm;
• the sorting chamber comprising:
• a base wall, which has a first inner surface;
• a top wall, which has a second inner surface and is connected in a fluid-tight way with the base wall;
• an internal chamber, which is at least partially delimited by the base wall and top wall;
• and
• at least one passage, which sets in communication the internal chamber with the external environment;
• wherein the base wall comprises:
• at least one cavity, which has at least one opening towards the internal chamber, and is closed at an end opposite the at least one opening such that the at least one cavity is closed towards the external environment, wherein the at least one cavity is adapted to receive and house at least one particle of the first type but not particles of the second type;
• and
• at least one wall or column that at least partially delimits the cavity and has a width of up to 5 μm;
• said at least one opening having a width of from 3 μm to 8 μm.