Decellularized pleural matrix
AssigneeEthicon, Inc.
InventorsSridevi Dhanaraj, Chandra M. Valmikinathan, Charito Buensuceso, Agnieszka Seyda
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Abstract
The present invention pertains to the development of biologically derived extracellular matrices (ECM) derived from decellularized pleura tissue. Such matrices are useful in many clinical and therapeutic applications, including the repair, reconstruction, sealing, or joining of tissue, tendons, bones, and/or ligaments. In addition, the present invention features methods of making a biologically derived ECM derived from decellularized pleura tissue. The invention further features laminated ECM matrices.
Claims24
A biologically derived extracellular matrix (ECM) construct comprising two surfaces and a plurality of layers of decellularized pleura tissue wherein each layer of tissue has a basement membrane surface and a serosal surface wherein said ECM construct is made by a method comprising the steps of:
- providing a pleura tissue;
- decellularizing the pleura tissue;
- forming a plurality of pleura tissue layers of the decellularized pleura tissue wherein each layer of decellularized pleura tissue has a basement membrane surface and a serosal surface:
- stacking the plurality of decellularized pleura tissue layers on top of each other forming a multi-layered stack;
- compressing the multi-layered stack under vacuum;
- lyophilizing the compressed multi-layered stack;
- and optionally rehydrating the lyophilized multi-layered stack to form said decellularized ECM construct.
A method of making a biologically derived extracellular matrix (ECM) construct, comprising the steps of:
- providing a pleura tissue;
- decellularizing the pleura tissue;
- forming a plurality of pleura tissue layers of the decellularized pleura tissue wherein each layer of decellularized pleura tissue has a basement membrane surface and a serosal surface:
- stacking the plurality of decellularized pleura tissue layers on top of each other forming a multi-layered stack;
- compressing the multi-layered stack under vacuum;
- lyophilizing the compressed multi-layered stack;
- and optionally rehydrating the lyophilized multi-layered stack to form said decellularized ECM construct.
Dependencies are inferred from the claim text.