Immunosuppressive treatments, formulations and methods
OwnerGustavo A. Garcia-Sánchez, María Josefa Bernad Bernadinventors
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Abstract
The present disclosure provides inventive methods and formulations for immunosuppressive therapy for dry eye and other related immune mediated eye diseases. The formulations can comprise one or more immunomodulatory drugs such as parenterally administered liposome encapsulated rapamycin and topically administered tacrolimus. Immunomodulatory drugs herein can be formulated and administered to address the causes and/or reduce the symptoms of dry eye and/or other related immune mediated diseases of the eye.
Claims9
A method of making an immunosuppressive formulation for administration to an eye by a modified heating method, comprising the steps of:
- hydrating cholesterol to create a cholesterol dispersion;
- hydrating lecithin and sucrose to create a lecithin dispersion;
- creating a mixture comprising the cholesterol dispersion, the lecithin dispersion, and rapamycin;
- and
- simultaneously heating and stirring the mixture to create a dispersion of liposomes loaded with rapamycin.
A method of making an immunosuppressive formulation for administration to an eye by a modified heating method, comprising the steps of:
- hydrating cholesterol to create a cholesterol dispersion;
- hydrating lecithin to create a lecithin dispersion;
- creating a mixture comprising the cholesterol dispersion, the lecithin dispersion, and rapamycin;
- and
- simultaneously heating and stirring the mixture to create a dispersion of liposomes loaded with rapamycin;
- wherein the mixture consists of:
- from approximately 0.04 to approximately 0.1% (g/ml) rapamycin;
- from approximately 0.077 to approximately 1.01% (g/ml) cholesterol;
- from approximately 0.23 to approximately 3.05% (g/ml) soybean lecithin;
- from approximately 0.92 to approximately 12.2% (g/ml) sucrose;
- approximately 2.4% (g/ml) glycerol;
- and
- phosphate buffered saline.
Dependencies are inferred from the claim text.