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US 9,801,713 B2Granted
Production of tissue engineered heart valves
AssigneeWake Forest University Health
InventorsAnthony Atala, James Yoo
Life of this patent
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Abstract
The invention is directed to methods for preparing artificial heart valves by preconditioning a matrix seeded with endothelial cells and smooth muscle cells differentiated from isolated progenitor cells. These cell seeded matrices are exposed to fluid conditions that mimic blood flow through the heart to produce tissue engineered heart valves that are analogous to native heart valves.
Claims9
A preconditioned tissue engineered heart valve, comprising:
- a biocompatible matrix seeded with at least one population of endothelial cells differentiated from progenitor cells ex vivo, wherein the seeded cells form at least one cell layer on the biocompatible matrix;
- a population of smooth muscle cells differentiated from progenitor cells and seeded onto the endothelial cell layer,
- wherein the biocompatible matrix is selected from the group consisting of a decellularized matrix, an electrospun matrix, and a synthetic polymer matrix, and
- wherein at least one cell of the population of endothelial cells, the population of smooth muscle cells, or a combination thereof comprise(s) an exogenous nucleic acid encoding an anti-inflammatory factor operably linked to a promoter.
1 more element
Dependencies are inferred from the claim text.