Recombinant strain producing L-amino acids, constructing method therefor and method for producing L-amino acids
AssigneeInstitute of Microbiology, Chinese Academy of Sciences
InventorsTingyi Wen, Xiuling Shang, Yun Zhang, Shuwen Liu, Yong Liang, Yu Zhang
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Abstract
The present invention relates to recombinant bacteria producing L-amino acid, in which the recombinant bacteria has reduced expression of the glucose-6-phosphate isomerase gene pgi and improved expression of the glucose-6-phosphate dehydrogenase gene -opcA than the starting bacteria, where the starting bacterium is a bacterial strain that can accumulate target amino acid(s) and preferably, the amino acid is L-histidine.
Claims20
A recombinant bacteria producing L-amino acid(s), said recombinant bacteria has reduced expression of the glucose-6-phosphate isomerase gene pgi and improved expression of the glucose-6-phosphate dehydrogenase gene zwf-opcA than the starting bacteria, wherein:
- said starting bacterium is a bacterial strain that can accumulate target amino acid(s) and preferably, said amino acid is L-histidine, wherein, the gene pgi on the chromosome of the recombinant bacterium has been inactivated, preferably knocked out or the regulatory element of the gene pgi has been replaced with a regulatory element with low transcription or low expression activity, also said recombinant bacteria has two or more copied genes zwf-opcA, or the promoter of the operon tkt-tal-zwf-opcA-devB on the chromosome of said starting bacterium is replaced with a strong promoter, preferably, said strong promoter is the promoter Peftu, of the original bacteria.
A method of constructing the recombinant bacteria capable of producing L-histidine, which comprises the following steps:
- reduce the expression of the glucose-6-phosphate isomerase Pgi in the starting bacteria and improve the expression of the glucose-6-phosphate dehydrogenase Zwf-OpcA in said starting bacteria to obtain said recombinant bacteria, wherein:
- said starting bacterium is a bacterial strain which can accumulate target amino acid(s), more preferably, said target L-amino acid.
Dependencies are inferred from the claim text.