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US 9,796,957 B2Granted
Genetically modified diazotrophs and methods of using same
AssigneeRegents of the University of Minnesota
InventorsBrett Barney, Carolann Marie Knutson, Mary Plunkett
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Abstract
This disclosure describes a genetically-modified diazotrophic microbe and methods that involve the genetically-modified diazotrophic microbe. Generally, the diazotrophic microbe is modified to excrete a nitrogen-containing compound in an amount greater than a comparable control diazotrophic microbe. The genetically-modified diazotrophic microbe can be co-cultured with a non-diazotroph and increase the growth of the non-diazotroph.
Claims4
A method of increasing growth of a non-diazotroph, the method comprising:
- co-culturing the non-diazotroph;
- and
- an Azotobacter spp. genetically modified to excrete a nitrogen-containing compound in an amount effective to support the growth of a non-diazotroph in co-culture, the genetic modification comprising:
- a deletion or disruption of at least a portion of ureABC;
- a deletion or disruption of at least a portion of amtB;
- or
- a deletion or disruption of at least a portion of nifA2;
- wherein the Azotobacter spp. is provided in an amount effective to increase the growth of the non-diazotroph compared to growth of the non-diazotroph in the absence of the diazotrophic microbe.
5 more elements
Dependencies are inferred from the claim text.