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US 9,796,770 B2Granted
Immunoglobulin reduced in thrombogenic agents and preparation thereof
AssigneeOmrix Biopharmaceuticals Ltd.
InventorsRoni Mintz, Oleg Belyaev, Israel Nur, Liliana Bar, Roberto Meidler
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Abstract
The invention relates to an immunoglobulin composition reduced in thrombogenic agents and to methods for its preparation. One method comprises subjecting an immunoglobulin containing solution to a negative cation exchanger chromatography at a pH in the range of higher than 3.8 to equal to or lower than 5.3. The solution can also be subjected to a negative anion exchanger chromatography at a pH in the range of 7 to 8.2.
Claims22
A method for removing thrombogenic agents selected from the group consisting of prekallikrein activator (PKA) and Factor XIa;
- PKA and Factor XI;
- PKA Factor XIa, and Kallikrein;
- PKA, Factor XI and Kallikrein;
- and PKA, Factor XIa, Factor XI and Kallikrein from an immunoglobulin containing solution, the method comprising the steps of:
- contacting the solution with a support comprising immobilized negatively charged groups equilibrated at a pH in the range of higher than 3.8 to equal to or lower than 5.3 and with a support comprising immobilized positively charged groups equilibrated at a pH in the range of 7.0 to 8.2 to allow PKA binding to the positively charged groups and Factor XIa, Factor XI and/or Kallikrein binding to the negatively charged groups;
- and collecting unbound fractions comprising immunoglobulin.
3 more elements
Dependencies are inferred from the claim text.