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US 9,795,667 B2Granted
Method of attenuating porcine pseudorabies virus, attenuated strains of porcine pseudorabies virus, vaccine composition and use thereof
AssigneePulike Biological Engineering, Inc.
InventorsKegong Tian, Feifei Tan, Jinzhong Sun, Xuke Zhang
Life of this patent
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Abstract
This invention provides a method of attenuating porcine pseudorabies virus, which can effectively and reproducibly attenuate porcine pseudorabies virus. The attenuated strain of porcine pseudorabies virus by use of said method can provide effective immunization for pigs.
Claims16
A method of attenuating porcine pseudorabies virus, comprising:
- (1) a step of cultivating the pseudorabies virus adapted to cell culture, wherein the pseudorabies virus is inoculated into subcultured mammalian cells, and then subcultured for at least five passages so as to obtain the porcine pseudorabies virus strain adapted to the subcultured mammalian cells;
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- (2) a step of attenuating the porcine pseudorabies virus, wherein the porcine pseudorabies virus strain adapted to the subcultured mammalian cells is inoculated into subcultured avian cells and then subcultured for at least one passage so as to obtain the attenuated strain of the porcine pseudorabies virus, wherein the genes of the attenuated strain of porcine pseudorabies virus have a serial deletion of 3455 bp starting from the 890th nucleotide of the gI gene.
Dependencies are inferred from the claim text.