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US 9,791,458 B2Granted
Dissolved protein arthritis markers
AssigneeThe University of British Columbia
InventorsAziz Ghahary, Wolodymyr Walter Peter Maksymowych, Ruhangiz Taghi Kilani
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Abstract
Methods and kits for diagnosing arthritis are provided. The methods may involve detection of 14-3-3 eta or gamma proteins in a sera or synovial fluid sample.
Claims9
A method for determining the efficacy of a therapeutic regimen in an arthritic patient, comprising:
- administering a therapeutic approach to a patient diagnosed with arthritis;
- obtaining a test biological sample from said patient, wherein said test sample is selected from the group consisting of blood, serum, plasma, and synovial fluid;
- detecting the presence or relative amount of the eta isoform of the 14-3-3 protein in the test sample;
- wherein said detection step comprises contacting the test sample with a non-human anti-human 14-3-3 protein antibody that is isoform specific for the 14-3-3 eta protein;
- and
- using the presence or relative amount of the 14-3-3 eta protein to establish the efficacy of the therapeutic regimen in said arthritic patient;
- wherein a decreased level of said 14-3-3 eta protein in said test sample in comparison with a previous sample from said patient is indicative of the efficacy of an ongoing therapeutic regimen.
4 more elements
A method of detecting the eta isoform of the 14-3-3 protein in a patient, said method comprising:
- a. obtaining a test sample from a patient at risk or suspected of having arthritis, wherein the test sample is selected from the group consisting of blood, serum, plasma, and synovial fluid;
- and
- b. detecting whether 14-3-3 eta protein is present in the test sample by contacting the test sample with an anti-14-3-3 eta protein antibody and detecting binding between 14-3-3 eta protein and the antibody.
Dependencies are inferred from the claim text.