Optogenetic probes for measuring membrane potential
AssigneePresident and Fellows of Harvard College
InventorsAdam E. Cohen, Joel Kralj, Adam D. Douglass
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Abstract
The invention provides methods, cells and constructs for optical measurement of membrane potential. These methods can be used in cells that are not accessible to presently available methods using electrodes. The methods can be directed to, for example, high-throughput drug screening assays to determine agents that can affect membrane potential of a target cell.
Claims22
An isolated and purified nucleic acid encoding Archaerhodopsin 3 with a mutation of D95 to reduce ion pumping activity compared to Arch 3 WT, the isolated and purified nucleic acid being operably linked to a nucleic acid encoding at least one additional fluorescent protein capable of indicating ion concentration in a cell.
An isolated cell comprising a nucleic acid encoding Archaerhodopsin 3 with a mutation at D95 to reduce ion pumping activity compared to Arch 3 WT and at least one additional fluorescent protein capable of indicating an ion concentration in the cell.
An isolated and purified nucleic acid encoding Archaerhodopsin 3 with a mutation of D95 to reduce ion pumping activity compared to Arch 3 WT, the isolated and purified nucleic acid being operably linked to a nucleic acid encoding at least one green fluorescent protein or a homolog thereof.
Dependencies are inferred from the claim text.