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US 9,791,447 B2Granted
Methods of identifying SENP1 inhibitors
AssigneeCity of Hope
InventorYuan Chen
Life of this patent
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Abstract
Provided herein are methods of detecting binding of an SENP1 polypeptide to a compound and methods for screening for inhibitors of SENP1. Further provided are aqueous compositions comprising SENP1 polypeptides and NMR apparatuses comprising the compositions for NMR analysis.
Claims10
A method of detecting binding of an SENP1 polypeptide to a compound, the method comprising:
- (i) contacting an SENP1-SUMO complex with a compound, wherein the SENP1-SUMO complex comprises an SENP1 polypeptide and a SUMO protein;
- (ii) allowing the compound to bind to the SENP1 polypeptide of the SENP1-SUMO complex, thereby forming a SENP1-SUMO-compound complex;
- (iii) detecting the SENP1-SUMO-compound complex using nuclear magnetic resonance, thereby detecting binding of the SENP1 polypeptide to the compound.
Dependencies are inferred from the claim text.