Electrochemical aptasensors with a gelatin B matrix
AssigneeUniversiteit Antwerpen
InventorsRonny J. P. Blust, Freddy Dardenne, Karolien De Wael, Lucien Nagels, Guido F. E. Van Camp
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Abstract
This invention provides: —an aptamer-based electrochemical sensor, wherein said aptamer is covalently bonded to or chemisorbed on an electrode, said aptamer forming a complex with a target molecule and is encapsulated by a gelatin B matrix; —a method of manufacturing said aptamer-based electrochemical sensor; —the use of the aptamer-based electrochemical sensor for the electrochemical determination of a concentration of a target molecule; and —a composite electrode combining a polymeric material and electrically conducting particles for selective analyte detection, wherein said electrode is coated with gelatin type B.
Claims13
An aptamer-based electrochemical sensor for detecting a target molecule, comprising an
- electrode, wherein an aptamer is covalently bonded to or chemisorbed on said electrode, said aptamer forming a complex with said target molecule, characterized in that said complex is encapsulated by a type B gelatin matrix.
A method of manufacturing an aptamer-based electrochemical sensor for determining a concentration of a target molecule comprising the steps of:
- selecting an aptamer to form a complex with a target molecule;
- synthesizing said aptamer;
- adsorbing said aptamer on, or covalently coupling said aptamer with, an electrode;
- and
- further providing a gelatin B matrix for said aptamer on said electrode.
A method for the electrochemical determination of a concentration of a target molecule, comprising the steps of:
- selecting an aptamer to form a complex with the target molecule;
- providing an electrode and a gelatin B matrix for said aptamer on said electrode, and
- adsorbing said aptamer on, or covalently coupling said aptamer to, said electrode.
Dependencies are inferred from the claim text.